Journal article
Hyaluronidase-inhibitory activities of glycosaminoglycans from Liparis tessellatus eggs
Gyeongsang National University1
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark2
Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark3
Biomass Conversion and Bioprocess Technology, Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark4
Changwon National University5
Polysaccharide fractions isolated from L. tessellatus eggs were purified and eluted using the DEAE-sepharose fast flow column. These were collected, tested and pooled based on their sugars content: F1, F2, and F3 which contain 26.8, 23.3, and 20.2% sulfated glycans; 34.5, 38.2, and 45.0% uronic acids; and 23.5, 19.0, and 7.5% acetylhexosamines and hexosamines, respectively.
Hyaluronidase inhibitory effects of the fractions are in the order F3>F2>F1>Ascorbic acid, with F3 having the highest inhibition among the fractions and that of the standard, ascorbic acid. The ESI-MS/MS confirmed the presence of uronic acids on F3, which could be a 0,2A2 fragment plus loss of methyl group which is very common among nonmethylated, sulfated disaccharides.
| Language: | English |
|---|---|
| Year: | 2017 |
| Pages: | 15-20 |
| ISSN: | 18791344 and 01448617 |
| Types: | Journal article |
| DOI: | 10.1016/j.carbpol.2016.12.065 |
| ORCIDs: | Mussatto, Solange Ines |
