Journal article
Assessment of flhDC mRNA levels in Serratia liquefaciens swarm cells
We reported previously that artificial overexpression of the flhDC operon in liquid-grown Serratia liquefaciens resulted in the formation of filamentous, multinucleated, and hyperflagellated cells that were indistinguishable from surface-induced swarm cells (L. Eberl, G. Christiansen, S. Molin, and M.
Givskov, J. Bacteriol. 178:554-559, 1996). In the present report we show by means of reporter gene measurements, Northern analysis, and in situ reverse transcription-PCR that the amount of flhDC mRNA in surface-grown swarm cells does not exceed the maximum level found in nondifferentiated, vegetative cells.
This suggests that surface-induced S. liquefaciens swarm cell differentiation, although dependent on flhDC gene expression, does not occur through elevated flhDC mRNA levels.
Language: | English |
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Publisher: | American Society for Microbiology |
Year: | 2000 |
Pages: | 2680-2686 |
ISSN: | 10985530 , 00219193 and 10678832 |
Types: | Journal article |
DOI: | 10.1128/JB.182.10.2680-2686.2000 |
ORCIDs: | Sternberg, Claus and Molin, Søren |