Journal article
Novel roles for the AIDA adhesin from diarrheagenic Escherichia coli:: Cell aggregation and biofilm formation
Diarrhea-causing Escherichia coli strains are responsible for numerous cases of gastrointestinal disease and constitute a serious health problem throughout the world. The ability to recognize and attach to host intestinal surfaces is an essential step in the pathogenesis of such strains. AIDA is a potent bacterial adhesin associated with some diarrheagenic E. coli strains.
AIDA mediates bacterial attachment to a broad variety of human and other mammalian cells. It is a surface-displayed autotransporter protein and belongs to the selected group of bacterial glycoproteins; only the glycosylated form binds to mammalian cells. Here, we show that AIDA possesses self-association characteristics and can mediate autoaggregation of E. coli cells.
We demonstrate that intercellular AIDA-AIDA interaction is responsible for bacterial autoaggregation. Interestingly, AIDA-expressing cells can interact with antigen 43 (Ag43) -expressing cells, which is indicative of an intercellular AIDA-Ag43 interaction. Additionally, AIDA expression dramatically enhances biofilm formation by E. coli on abiotic surfaces in How chambers.
Language: | English |
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Publisher: | American Society for Microbiology |
Year: | 2004 |
Pages: | 8058-8065 |
ISSN: | 10985530 , 00219193 and 10678832 |
Types: | Journal article |
DOI: | 10.1128/JB.186.23.8058-8065.2004 |