Journal article
Diagnostic PCR: Comparative sensitivity of four probe chemistries
In
Molecular and Cellular Probes
—
2009, Volume 23, Issue 3-4, pp. 201-203
Abstract
Three probe chemistries: locked nucleic acid (LNA), minor groove binder (MGB) and Scorpion were compared with a TaqMan probe in a validated real-time PCR assay for detection of food-borne thermotolerant Campylobacter. The LNA probe produced significantly lower Ct-values and a higher proportion of positive PCR responses analyzing less than 150 DNA copies than the TaqMan probe.
Choice of probe chemistry clearly has an impact on the sensitivity of PCR assays, and should be considered in an optimization strategy.
| Language: | English |
|---|---|
| Year: | 2009 |
| Pages: | 201-203 |
| ISSN: | 10961194 and 08908508 |
| Types: | Journal article |
| DOI: | 10.1016/j.mcp.2009.02.003 |
| ORCIDs: | Löfström, Charlotta and Hoorfar, Jeffrey |
