Journal article
Stable and high-yielding intrinsic 59Fe-radiolabeling of the intravenous iron preparations Monofer and Cosmofer: Intrinsic 59Fe-radiolabeling of Monofer and Cosmofer : Intrinsic 59Fe-radiolabeling of Monofer and Cosmofer
Commercial iron supplements Monofer((R)) and Cosmofer((R)) were intrinsically radiolabeled with Fe-59 for the purpose of tracing iron absorption in vivo. Optimized procedures aimed at introducing Fe-59 into the macromolecular construct in a form that was as chemically equivalent to the matrix iron as possible.
This was determined by challenging the labeled constructs with diethylenetriaminepentaacetic acid (DTPA) followed by separation by size-exclusion and measurements of radioactivity and iron in the eluted fractions. The final procedures were simple and involved heating aqueous dispersions of the supplements in the presence of [Fe-59]FeCl3 for 24h at 95 degrees C for Monofer, and 85 degrees C for Cosmofer, resulting in radiochemical yields greater than 94%.
High performance size exclusion chromatography, UV-VIS spectroscopy, and dynamic light scattering were used to show that the supplements remained unchanged after radiolabeling, underscoring the applicability of the methodology for radiolabeling commercial iron preparations.
Language: | English |
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Year: | 2016 |
Pages: | 375-382 |
ISSN: | 03624803 and 10991344 |
Types: | Journal article |
DOI: | 10.1002/jlcr.3416 |
ORCIDs: | Jensen, Andreas Tue Ingemann and Severin, Gregory |