Journal article
Confinement Spectroscopy: Probing Single DNA Molecules with Tapered Nanochannels
NSE-Optofluidics Group, NanoSystemsEngineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark1
NanoSystemsEngineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark2
Department of Micro- and Nanotechnology, Technical University of Denmark3
Polymer Microsystems for Cell Processing Group, Polymer Micro and Nano Engineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark4
Polymer Micro and Nano Engineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark5
We demonstrate a confinement spectroscopy technique capable of probing small conformational changes of unanchored single DNA molecules in a manner analogous to force spectroscopy, in the regime corresponding to femtonewton forces. In contrast to force spectroscopy, various structural forms of DNA can easily be probed, as indicated by experiments on linear and circular DNA.
The extension of circular DNA is found to scale according to the de Gennes exponent, unlike for linear DNA.
Language: | English |
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Year: | 2009 |
Pages: | 1382-1385 |
ISSN: | 15306992 and 15306984 |
Types: | Journal article |
DOI: | 10.1021/nl803030e |
ORCIDs: | Larsen, Niels Bent and Kristensen, Anders |