Journal article
Measurement of membrane elasticity by micro-pipette aspiration
Colloids and Biological Interfaces Group, Self-organizing materials for nanotechnology Section, Department of Micro- and Nanotechnology, Technical University of Denmark1
Self-organizing materials for nanotechnology Section, Department of Micro- and Nanotechnology, Technical University of Denmark2
Department of Micro- and Nanotechnology, Technical University of Denmark3
University of Southern Denmark4
The classical micro-pipette aspiration technique, applied for measuring the membrane bending elasticity, is in the present work reviewed and extended to span the range of pipette aspiration pressures going through the °accid (low pressures) to tense (high pressures) membrane regime. The quality of the conventional methods for analysing data is evaluated using numerically generated data and a new method for data analysis, based on thermodynamic analysis and detailed statistical mechanical modelling, is introduced.
The analysis of the classical method, where the membrane bending modulus is obtained from micro-pipette aspiration data acquired in the low-pressure regime, reveals a signi¯cant correction from membrane stretching elasticity. The new description, which includes the full vesicle geometry and both the membrane bending and stretching elasticity, is used for the interpretation of micro-pipette aspiration experiments conducted on SOPC (stearoyl-oleoyl-phosphatidyl-choline) lipid vesicles in the °uid phase.
The data analysis, which is extended by detailed image analysis and a ¯tting procedure based on Monte Carlo integration, gives an estimate of the bending modulus, that agrees with previously published results obtained by the use of shape °uctuation analysis of giant unilamellar vesicles. The obtained estimate of the area expansion modulus, is automatically corrected for contributions from residual thermal undulations and the equilibrium area of the vesicle is resolved.
Language: | English |
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Publisher: | Springer-Verlag |
Year: | 2004 |
Pages: | 149-167 |
ISSN: | 1292895x and 12928941 |
Types: | Journal article |
DOI: | 10.1140/epje/i2003-10146-y |
ORCIDs: | Henriksen, Jonas Rosager |
1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine Aspiration Technique Computer Simulation Elasticity Giant Unilamellar Vesicle Lipid Bilayers Lipid Vesicle Liposomes Membrane Fluidity Membranes, Artificial Microfluidics Micromanipulation Models, Chemical Monte Carlo Integration Phosphatidylcholines Physics Pressure Statistical Mechanical Modelling Stress, Mechanical Surface Tension Vacuum