Journal article
Modulation of Cytokine mRNA Expression in Pharyngeal Epithelial Samples obtained from Cattle Infected with Foot-and-Mouth Disease Virus
Sektion for Eksotiske Virussygdomme, Division of Virology, National Veterinary Institute, Technical University of Denmark1
Division of Virology, National Veterinary Institute, Technical University of Denmark2
National Veterinary Institute, Technical University of Denmark3
Innate Immunology, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark4
Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark5
DTU Data Analysis, Department of Informatics and Mathematical Modeling, Technical University of Denmark6
A novel technique of endoscopical collection of small tissue samples was used to obtain sequential tissue samples from the dorsal soft palate (DSP) of individual cattle infected with foot-and-mouth disease virus (FMDV) at different phases of the infection. Levels of mRNA encoding interferon (IFN)-a and IFN-b as well as tumour necrosis factor (TNF)-a were measured in these samples by quantitative reverse transcriptase polymerase chain reaction.
Expression of IFN-b mRNA was significantly down-regulated in the biopsy samples harvested during the acute phase of infection, while there was no statistically significant effect on the expression of IFN-a mRNA compared with baseline levels. In contrast, the mRNA encoding TNF-a was significantly up-regulated in samples collected during both acute and late (>28 days post infection) phases of infection.
There were also significantly higher levels of TNF-a mRNA expressed in samples derived from animals that were identified subsequently as persistently infected FMDV-carriers. It was concluded that there was a significant difference in the host-response in the DSP of calves that were identified as persistently infected, subclinical carriers of FMDV.
Language: | English |
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Year: | 2012 |
Pages: | 243-252 |
ISSN: | 15323129 and 00219975 |
Types: | Journal article |
DOI: | 10.1016/j.jcpa.2011.06.005 |
ORCIDs: | Belsham, Graham , Heegaard, Peter M. H. and Stockmarr, Anders |
Animals Cattle Cattle Diseases Cattle Diseases/genetics Foot-and-Mouth Disease Foot-and-Mouth Disease Virus Foot-and-Mouth Disease Virus/genetics Foot-and-Mouth Disease/genetics Interferon-alpha Interferon-alpha/genetics Interferon-beta Interferon-beta/genetics Pharynx Pharynx/metabolism RNA, Messenger RNA, Messenger/genetics Tumor Necrosis Factor-alpha Tumor Necrosis Factor-alpha/genetics cytokine mRNA foot-and-mouth disease pharyngeal epithelium