Journal article
Gold Nanoparticles-Coated SU-8 for Sensitive Fluorescence-Based Detections of DNA
Department of Micro- and Nanotechnology, Technical University of Denmark1
University of Southampton2
National Veterinary Institute, Technical University of Denmark3
Section for Public sector service and commercial diagnostics, National Veterinary Institute, Technical University of Denmark4
BioLabChip, Department of Micro- and Nanotechnology, Technical University of Denmark5
National Food Institute, Technical University of Denmark6
Division of Food Microbiology, National Food Institute, Technical University of Denmark7
SU-8 epoxy-based negative photoresist has been extensively employed as a structural material for fabrication of numerous biological microelectro-mechanical systems (Bio-MEMS) or lab-on-a-chip (LOC) devices. However, SU-8 has a high autofluorescence level that limits sensitivity of microdevices that use fluorescence as the predominant detection workhorse.
Here, we show that deposition of a thin gold nanoparticles layer onto the SU-8 surface significantly reduces the autofluorescence of the coated SU-8 surface by as much as 81% compared to bare SU-8. Furthermore, DNA probes can easily be immobilized on the Au surface with high thermal stability. These improvements enabled sensitive DNA detection by simple DNA hybridization down to 1 nM (a two orders of magnitude improvement) or by solid-phase PCR with sub-picomolar sensitivity.
The approach is simple and easy to perform, making it suitable for various Bio-MEMs and LOC devices that use SU-8 as a structural material.
Language: | English |
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Publisher: | MDPI |
Year: | 2012 |
Pages: | 72-82 |
ISSN: | 20754418 |
Types: | Journal article |
DOI: | 10.3390/diagnostics2040072 |
ORCIDs: | Wolff, Anders and Bang, Dang Duong |