Journal article
Engineered CHO cells for production of diverse, homogeneous glycoproteins
Technical University of Denmark1
University of Copenhagen2
Department of Systems Biology, Technical University of Denmark3
Novo Nordisk Foundation4
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark5
Glyco-Engineering of CHO, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark6
Production of glycoprotein therapeutics in Chinese hamster ovary (CHO) cells is limited by the cells' generic capacity for N-glycosylation, and production of glycoproteins with desirable homogeneous glycoforms remains a challenge. We conducted a comprehensive knockout screen of glycosyltransferase genes controlling N-glycosylation in CHO cells and constructed a design matrix that facilitates the generation of desired glycosylation, such as human-like alpha 2,6-linked sialic acid capping.
This engineering approach will aid the production of glycoproteins with improved properties and therapeutic potential.
| Language: | English |
|---|---|
| Publisher: | Nature Publishing Group US |
| Year: | 2015 |
| Pages: | 842-844 |
| Journal subtitle: | Science and Business of Biotechnology |
| ISSN: | 15461696 and 10870156 |
| Types: | Journal article |
| DOI: | 10.1038/nbt.3280 |
| ORCIDs: | 0000-0003-1629-187X , 0000-0003-3691-9820 , 0000-0002-4976-0647 , 0000-0003-0240-9232 and 0000-0002-0915-5055 |
