Journal article
Structural features of peptoid-peptide hybrids in lipid-water interfaces
Department of Biotechnology, Chemistry, and Environmental Engineering, Aalborg University, Sohngaardsholmsvej 49, 9000 Aalborg, Denmark.1
Department of Drug Design and Pharmacology, University of Copenhagen, Universitetsparken 2, 2100 Copenhagen, Denmark.2
Institute of Biophysical Chemistry, Center for Biomolecular Magnetic Resonance, J. W. Goethe-University Frankfurt, Max-von-Laue-Str. 9, 60438 Frankfurt am Main, Germany.3
Department of Biotechnology, Chemistry, and Environmental Engineering, Aalborg University, Sohngaardsholmsvej 49, 9000 Aalborg, Denmark. Electronic address: rw@bio.aau.dk.4
The inclusion of peptoid monomers into antimicrobial peptides (AMPs) increases their proteolytic resistance, but introduces conformational flexibility (reduced hydrogen bonding ability and cis/trans isomerism). We here use NMR spectroscopy to answer how the insertion of a peptoid monomer influences the structure of a regular α-helical AMP upon interaction with a dodecyl phosphocholine (DPC) micelle.
Insertion of [(2-methylpropyl)amino]acetic acid in maculatin-G15 shows that the structural change and conformational flexibility depends on the site of insertion. This is governed by the micelle interaction of the amphipathic helices flanking the peptoid monomer and the side chain properties of the peptoid and its preceding residue.
Language: | English |
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Year: | 2014 |
Pages: | 3291-3297 |
ISSN: | 18733468 and 00145793 |
Types: | Journal article |
DOI: | 10.1016/j.febslet.2014.07.016 |
ORCIDs: | Wimmer, Reinhard and 0000-0001-9357-8001 |
AMP Amino Acid Sequence Antimicrobial Cationic Peptides Antimicrobial peptides CCA COSY DIPCDI DPC Fmoc Gd(DTPA-BMA) HPLC HSQC Lipids Maculatin Models, Molecular Molecular Sequence Data NMR NOE NOESY Nleu PRE Paramagnetic relaxation enhancement Peptoids Phosphorylcholine Protein Conformation RMSD SDS TFA TFE TIS TOCSY Water [(2-methylpropyl)amino]acetic acid antimicrobial peptide correlation spectroscopy diisopropylcarbodiimide dodecyl phosphocholine dodecylphosphocholine fluorenylmethyloxycarbonyl chloride gadolinium diethylenetriaminepentaacetic acid bismethylamide heteronuclear single quantum coherence high pressure liquid chromatography nuclear Overhauser effect nuclear Overhauser effect spectroscopy paramagnetic relaxation enhancement root mean square deviation sodium dodecyl sulfate total correlation spectroscopy trifluoroacetic acid trifluoroethanol triisopropyl silane α-cyano-4-hydroxycinnamic acid