Journal article
Impact of Rap-Phr system abundance on adaptation of Bacillus subtilis
Friedrich Schiller University Jena1
Biotherapeutic Engineering and Drug Targeting, Department of Health Technology, Technical University of Denmark2
Colloids & Biological Interfaces, Biotherapeutic Engineering and Drug Targeting, Department of Health Technology, Technical University of Denmark3
Center for Microbial Secondary Metabolites, Centers, Technical University of Denmark4
Department of Biotechnology and Biomedicine, Technical University of Denmark5
Section for Microbial and Chemical Ecology, Department of Biotechnology and Biomedicine, Technical University of Denmark6
Technical University of Denmark7
SeqOmics Biotechnology Ltd.8
Hungarian Academy of Sciences9
National Centre for Nano Fabrication and Characterization, Technical University of Denmark10
Department of Health Technology, Technical University of Denmark11
Nanocharacterization, National Centre for Nano Fabrication and Characterization, Technical University of Denmark12
...and 2 moreMicrobes commonly display great genetic plasticity, which has allowed them to colonize all ecological niches on Earth. Bacillus subtilis is a soil-dwelling organism that can be isolated from a wide variety of environments. An interesting characteristic of this bacterium is its ability to form biofilms that display complex heterogeneity: individual, clonal cells develop diverse phenotypes in response to different environmental conditions within the biofilm.
Here, we scrutinized the impact that the number and variety of the Rap-Phr family of regulators and cell-cell communication modules of B. subtilis has on genetic adaptation and evolution. We examine how the Rap family of phosphatase regulators impacts sporulation in diverse niches using a library of single and double rap-phr mutants in competition under 4 distinct growth conditions.
Using specific DNA barcodes and whole-genome sequencing, population dynamics were followed, revealing the impact of individual Rap phosphatases and arising mutations on the adaptability of B. subtilis.
| Language: | English |
|---|---|
| Publisher: | Nature Publishing Group UK |
| Year: | 2021 |
| Pages: | 468 |
| ISSN: | 23993642 |
| Types: | Journal article |
| DOI: | 10.1038/s42003-021-01983-9 |
| ORCIDs: | 0000-0002-2366-0912 , Christensen, Mathilde Nordgaard , 0000-0002-5174-1146 , Kempen, Paul J. , Dragoš, Anna and Kovács, Ákos T. |
