Journal article
Investigation of inter- and intraspecies variation through genome sequencing of Aspergillus section Nigri
Department of Biotechnology and Biomedicine, Technical University of Denmark1
Fungal Biomedicine and Biology, Section for Synthetic Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark2
Eukaryotic Molecular Cell Biology, Section for Synthetic Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark3
Section for Synthetic Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark4
Network Engineering of Eukaryotic Cell factories, Section for Synthetic Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark5
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark6
New Bioactive Compounds, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark7
Center for Microbial Secondary Metabolites, Centers, Technical University of Denmark8
Fungal Chemodiversity, Section for Microbial and Chemical Ecology, Department of Biotechnology and Biomedicine, Technical University of Denmark9
Section for Microbial and Chemical Ecology, Department of Biotechnology and Biomedicine, Technical University of Denmark10
Natural Product Discovery, Section for Microbial and Chemical Ecology, Department of Biotechnology and Biomedicine, Technical University of Denmark11
...and 1 moreAspergillus section Nigri comprises filamentous fungi relevant to biomedicine, bioenergy, health, and biotechnology. To learn more about what genetically sets these species apart, as well as about potential applications in biotechnology and biomedicine, we sequenced 23 genomes de novo, forming a full genome compendium for the section (26 species), as well as 6 Aspergillus niger isolates.
This allowed us to quantify both inter- and intraspecies genomic variation. We further predicted 17,903 carbohydrate-active enzymes and 2,717 secondary metabolite gene clusters, which we condensed into 455 distinct families corresponding to compound classes, 49% of which are only found in single species.
We performed metabolomics and genetic engineering to correlate genotypes to phenotypes, as demonstrated for the metabolite aurasperone, and by heterologous transfer of citrate production to Aspergillus nidulans. Experimental and computational analyses showed that both secondary metabolism and regulation are key factors that are significant in the delineation of Aspergillus species.
| Language: | English |
|---|---|
| Publisher: | Nature Publishing Group US |
| Year: | 2018 |
| Pages: | 1688-1695 |
| ISSN: | 15461718 and 10614036 |
| Types: | Journal article |
| DOI: | 10.1038/s41588-018-0246-1 |
| ORCIDs: | Nybo, Jane L , 0000-0001-7042-8326 , Frisvad, Jens Christian , Larsen, Thomas Ostenfeld , Nielsen, Kristian Fog , 0000-0001-7712-7024 , 0000-0002-1332-1810 , 0000-0003-0771-2329 , 0000-0002-3136-8903 , Mortensen, Uffe Hasbro , Andersen, Mikael Rørdam , Vesth, Tammi Camilla , Hoof, Jakob Blæsbjerg and Brandl, Julian |
