Journal article
Orally active bivalent VHH construct prevents proliferation of F4+ enterotoxigenic Escherichia coli in weaned piglets
Technical University of Denmark1
Infection Microbiology, Section for Microbial and Chemical Ecology, Department of Biotechnology and Biomedicine, Technical University of Denmark2
Center for Microbial Secondary Metabolites, Centers, Technical University of Denmark3
Aarhus University4
Disease Systems Immunology, Section for Protein Science and Biotherapeutics, Department of Biotechnology and Biomedicine, Technical University of Denmark5
Bactolife ApS6
Department of Biotechnology and Biomedicine, Technical University of Denmark7
Section for Protein Chemistry and Enzyme Technology, Department of Biotechnology and Biomedicine, Technical University of Denmark8
Protein Glycoscience and Biotechnology, Section for Protein Chemistry and Enzyme Technology, Department of Biotechnology and Biomedicine, Technical University of Denmark9
DTU Microbes Initiative, Centers, Technical University of Denmark10
Section for Protein Science and Biotherapeutics, Department of Biotechnology and Biomedicine, Technical University of Denmark11
Tropical Pharmacology and Biotherapeutics, Section for Protein Science and Biotherapeutics, Department of Biotechnology and Biomedicine, Technical University of Denmark12
Section for Microbial and Chemical Ecology, Department of Biotechnology and Biomedicine, Technical University of Denmark13
...and 3 moreA major challenge in industrial pig production is the prevalence of post-weaning diarrhea (PWD) in piglets, often caused by enterotoxigenic Escherichia coli (ETEC). The increased use of antibiotics and zinc oxide to treat PWD has raised global concerns regarding antimicrobial resistance development and environmental pollution.
Still, alternative treatments targeting ETEC and counteracting PWD are largely lacking. Here, we report the design of a pH, temperature, and protease-stable bivalent VHH-based protein BL1.2 that cross-links a F4+ ETEC model strain by selectively binding to its fimbriae. This protein inhibits F4+ ETEC adhesion to porcine epithelial cells ex vivo and decreases F4+ ETEC proliferation when administrated as a feed additive to weaned F4+ ETEC challenged piglets.
These findings highlight the potential of a highly specific bivalent VHH-based feed additive in effectively delimiting pathogenic F4+ ETEC bacteria proliferation in piglets and may represent a sustainable solution for managing PWD while circumventing antimicrobial resistance development.
| Language: | English |
|---|---|
| Publisher: | Elsevier |
| Year: | 2022 |
| Pages: | 104003 |
| ISSN: | 25890042 |
| Types: | Journal article |
| DOI: | 10.1016/j.isci.2022.104003 |
| ORCIDs: | Pichler, Michael , Ledsgaard, Line , Ahmadi, Shirin , Hermansen, Grith Miriam Maigaard , Jelsbak, Lars , Brix, Susanne and Laustsen, Andreas Hougaard |
Infection control in health technology Microbiology Porcine medicine Q Science
