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Journal article

The Use of Infrared Thermography as a Novel Approach for Real-Time Validation of PCR Thermocyclers

From

National Food Institute, Technical University of Denmark1

Division of Food Microbiology, National Food Institute, Technical University of Denmark2

Validation of PCR thermocycler performance is crucial to obtain reliable results. In this study, infrared (IR) thermography was evaluated as a novel validation tool. After stabilisation, no significant difference in the temperatures recorded using thermography and a reference blockbased system was found.

By employing IR thermography, information about the length of the time until temperature stabilisation in the sample could be obtained. This study shows the potential of using IR thermography for validation of thermocyclers.

Language: English
Publisher: Springer-Verlag
Year: 2010
Pages: 116-119
ISSN: 1936976x and 19369751
Types: Journal article
DOI: 10.1007/s12161-009-9103-2
ORCIDs: Löfström, Charlotta and Hoorfar, Jeffrey

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