Journal article
A comparative genomics study of 23 Aspergillus species from section Flavi
Department of Biotechnology and Biomedicine, Technical University of Denmark1
Joint Bioenergy Institute2
Aix-Marseille Université3
Eukaryotic Molecular Cell Biology, Section for Synthetic Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark4
Center for Microbial Secondary Metabolites, Centers, Technical University of Denmark5
Kanazawa Institute of Technology6
Section for Synthetic Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark7
Fungal Chemodiversity, Section for Microbial and Chemical Ecology, Department of Biotechnology and Biomedicine, Technical University of Denmark8
Section for Microbial and Chemical Ecology, Department of Biotechnology and Biomedicine, Technical University of Denmark9
Natural Product Discovery, Section for Microbial and Chemical Ecology, Department of Biotechnology and Biomedicine, Technical University of Denmark10
Joint Genome Institute11
Kikkoman Corporation12
Utrecht University13
University of Helsinki14
...and 4 moreSection Flavi encompasses both harmful and beneficial Aspergillus species, such as Aspergillus oryzae, used in food fermentation and enzyme production, and Aspergillus flavus, food spoiler and mycotoxin producer. Here, we sequence 19 genomes spanning section Flavi and compare 31 fungal genomes including 23 Flavi species.
We reassess their phylogenetic relationships and show that the closest relative of A. oryzae is not A. flavus, but A. minisclerotigenes or A. aflatoxiformans and identify high genome diversity, especially in sub-telomeric regions. We predict abundant CAZymes (598 per species) and prolific secondary metabolite gene clusters (73 per species) in section Flavi.
However, the observed phenotypes (growth characteristics, polysaccharide degradation) do not necessarily correlate with inferences made from the predicted CAZyme content. Our work, including genomic analyses, phenotypic assays, and identification of secondary metabolites, highlights the genetic and metabolic diversity within section Flavi.
| Language: | English |
|---|---|
| Publisher: | Nature Publishing Group UK |
| Year: | 2020 |
| Pages: | 1106 |
| ISSN: | 20411723 |
| Types: | Journal article |
| DOI: | 10.1038/s41467-019-14051-y |
| ORCIDs: | Frisvad, Jens Christian , 0000-0003-0771-2329 , 0000-0002-0229-0975 , 0000-0002-5838-1972 , 0000-0002-1332-1810 , 0000-0001-7712-7024 , Mortensen, Uffe Hasbro , Larsen, Thomas Ostenfeld , 0000-0002-4363-1123 , 0000-0002-3136-8903 , 0000-0001-5085-3106 , Andersen, Mikael R. , Kjærbølling, Inge , Vesth, Tammi Camilla , Nybo, Jane L. , Kildgaard, Sara and Petersen, Thomas Isbrandt |
Applied microbiology Aspergillus flavus Aspergillus oryzae Bioreactors Carbohydrate Metabolism Crops, Agricultural DNA, Fungal Fermentation Fermented Foods Fungal Proteins Fungal genomics Genome, Fungal Genomics Industrial microbiology Metabolic Networks and Pathways Multigene Family Phenotype Phylogeny Plant Diseases Q Science Secondary Metabolism Small molecules
