Journal article
A novel biomarker of MMP-cleaved prolargin is elevated in patients with psoriatic arthritis
Nordic Bioscience AS1
Lund University2
Aalborg University Hospital3
Department of Biotechnology and Biomedicine, Technical University of Denmark4
Protease Network Degradomics, Section for Protein Science and Biotherapeutics, Department of Biotechnology and Biomedicine, Technical University of Denmark5
Psoriatic arthritis (PsA) is a chronic musculoskeletal inflammatory disease found in up to 30% of psoriasis patients. Prolargin—an extracellular matrix (ECM) protein present in cartilage and tendon—has been previously shown elevated in serum of patients with psoriasis. ECM protein fragments can reflect tissue turnover and pathological changes; thus, this study aimed to develop, validate and characterize a novel biomarker PROM targeting a matrix metalloproteinase (MMP)-cleaved prolargin neo-epitope, and to evaluate it as a biomarker for PsA.
A competitive ELISA was developed with a monoclonal mouse antibody; dilution- and spiking-recovery, inter- and intra-variation, and accuracy were evaluated. Serum levels were evaluated in 55 healthy individuals and 111 patients diagnosed with PsA by the CASPAR criteria. Results indicated that the PROM assay was specific for the neo-epitope.
Inter- and intra- assay variations were 11% and 4%, respectively. PROM was elevated (p = 0.0003) in patients with PsA (median: 0.24, IQR: 0.19–0.31) compared to healthy controls (0.18; 0.14–0.23) at baseline. AUROC for separation of healthy controls from PsA patients was 0.674 (95% CI 0.597–0.744, P <0.001).
In conclusion, MMP-cleaved prolargin can be quantified in serum by the PROM assay and has the potential to separate patients with PsA from healthy controls.
| Language: | English |
|---|---|
| Publisher: | Nature Publishing Group UK |
| Year: | 2020 |
| Pages: | 13541 |
| ISSN: | 20452322 |
| Types: | Journal article |
| DOI: | 10.1038/s41598-020-70327-0 |
AC133 Antigen Animals Antibodies, Monoclonal Antibody Formation Arthritis, Psoriatic Biomarkers Case-Control Studies Cohort Studies DELAY DIAGNOSIS DISEASE ELISA Enzyme-Linked Immunosorbent Assay Extracellular Matrix Proteins Female Glycoproteins Humans Male Matrix Metalloproteinases Mice Mice, Inbred BALB C Middle Aged PRELP PRELP protein, human PROM1 protein, human Psoriatic arthritis RICH REPEAT PROTEIN ROC Curve TIMP-1
