Journal article
Gcn4p and the Crabtree effect of yeast: drawing the causal model of the Crabtree effect in Saccharomyces cerevisiae and explaining evolutionary trade-offs of adaptation to galactose through systems biology
Department of Chemical and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.1
By performing an integrated comparative analysis on the physiology and transcriptome of four different S. cerevisiae strains growing on galactose and glucose, it was inferred that the transcription factors Bas1p, Pho2p, and Gcn4p play a central role in the regulatory events causing the Crabtree effect in S. cerevisiae.
The analysis also revealed that a point mutation in the RAS2 observed in a galactose-adapted strain causes a lower Crabtree effect and growth rate on glucose by decreasing the activity of Gcn4p while at the same time is at the origin of higher growth rate on galactose due to a lower activity of the transcriptional repressor Sok2p.
The role of Gcn4p on the trade-off effect observed on glucose was confirmed experimentally. This was done by showing that the point mutation in RAS2 does not result in a lower growth rate on glucose if it is introduced in a GCN4-negative background.
Language: | English |
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Publisher: | Oxford University Press (OUP) |
Year: | 2014 |
Pages: | 654-62 |
ISSN: | 15671356 and 15671364 |
Types: | Journal article |
DOI: | 10.1111/1567-1364.12153 |
Adaptation, Physiological BAS1 protein, S cerevisiae Bas1 Basic-Leucine Zipper Transcription Factors Crabtree effect GCN4 protein, S cerevisiae Galactose Gcn4 Gene Expression Profiling Glucose Homeodomain Proteins Metabolic Networks and Pathways PHO2 protein, S cerevisiae Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Systems Biology Trans-Activators flux analysis random sampling