Conference paper
Thin-film Composite Biocatalytic Nanofibrous Membrane System for Pharmaceuticals’ Degradation: Performance, stability, and Continuity
Water Technology & Processes, Department of Environmental Engineering, Technical University of Denmark1
Department of Environmental Engineering, Technical University of Denmark2
Protein Glycoscience and Biotechnology, Section for Protein Chemistry and Enzyme Technology, Department of Biotechnology and Biomedicine, Technical University of Denmark3
Department of Biotechnology and Biomedicine, Technical University of Denmark4
Recently, biocatalytic nanofibrous membranes have drawn attention in various fields such as biosensing and wastewater treatment because of their high specificity, prolonged reusability and low-cost efficiency. This study investigates a facile laccase-assisted nanofibrous filtration membrane system for transformation of pharmaceuticals.
Laccases are successfully covalently immobilized on functionalized electrospun polyacrylonitile/β-cyclodextrin nanofibers. β-Cyclodextrin (β-CD), a cone-shape cyclic hepta-α-1,4-glucosyl compound, whose apolar cavity can encapsulate a variety of hydrophobic guest molecules. Intrestingly, β-CD offer a functional and spatial scaffold for enzyme immobilization.
The catalytic kinetics parameters of both free and immobilized laccases are investigated. The immobilized enzyme shows a weaker affinity to the substrate but vastly improved stability compared to the free form, indicating that the immobilization elicits a considerable descrease in the rate of irreversible activation of the enzyme.
The biocatalytic membrane can be reused and retains up to 90% of the initial activity after five cycles. Inspired by the improved stability, the enzymatic membranes combined with thin-film composite membrane are furthered investigated in a cross-flow filtration system to achieve a continuous and efficient removal of mefenamic acid.
The removal of mefenamic acid could be maintained at 90%. Our findings demonstrate that laccase-assisted electrospun materials have the potential for application in environmental protection processes.
Language: | English |
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Year: | 2021 |
Proceedings: | Membrane Desalination 2021 |
Types: | Conference paper |
ORCIDs: | Zhao, Dan , Leth, Maria Louise , Hachem, Maher Abou , Hélix-Nielsen, Claus and Zhang, Wenjing (Angela) |