Journal article
A Semi-Closed Device for Chromosome Spreading for Cytogenetic Analysis
Department of Micro- and Nanotechnology, Technical University of Denmark1
Nano Bio Integrated Systems, Department of Micro- and Nanotechnology, Technical University of Denmark2
Amphiphilic Polymers in Biological Sensing, Department of Micro- and Nanotechnology, Technical University of Denmark3
Universidad Autónoma de Madrid4
University of Copenhagen5
Copenhagen University Hospital Herlev and Gentofte6
Metaphase chromosome spreading is the most crucial step required for successful karyotyping and FISH analysis. These two techniques are routinely used in cytogenetics to assess the chromosome abnormalities. The spreading process has been studied for years but it is still considered an art more than a science.
The chromosome spreading greatly depends on the environmental conditions such as humidity and temperature, which govern the evaporation of fixative, in which the cells are suspended. The spreading is normally performed manually in ambient conditions on glass slides, which are hydrophilic, and thus allow for better quality spreads.
Further cytogenetic analysis depends on the quality of the spreads, which is dependent on the skills of the personnel and is thus limited to laboratory settings. Here, we present a semi-closed microfluidic chip for preparation of the metaphase spreads on a glass and a Topasr substrate rendered more hydrophilic by oxygen plasma treatment coupled with photografting.
The device consists of a microfluidic chamber with perfusion holes that facilitate the evaporation of fixative and reliable formation of the spreads. The usability of the chromosome spreads formed on the glass and the Topasr slide is tested by performing FISH analysis.
Language: | English |
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Publisher: | MDPI AG |
Year: | 2014 |
Pages: | 158-170 |
ISSN: | 2072666x |
Types: | Journal article |
DOI: | 10.3390/mi5020158 |
ORCIDs: | Dimaki, Maria , Almdal, Kristoffer and Svendsen, Winnie Edith |