Journal article
Immobilization of BMP-2 and VEGF within Multilayered Polydopamine-Coated Scaffolds and the Resulting Osteogenic and Angiogenic Synergy of Co-Cultured Human Mesenchymal Stem Cells and Human Endothelial Progenitor Cells
Department of Health Technology, Technical University of Denmark1
CSIC - Institute of Materials Science of Barcelona2
Biomimetrics, Biocarriers and Bioimplants, Immunobiology and Biomimetics, Department of Health Technology, Technical University of Denmark3
Immunobiology and Biomimetics, Department of Health Technology, Technical University of Denmark4
We have previously reported the fabrication of a polycaprolactone and hydroxyapatite composite scaffold incorporating growth factors to be used for bone regeneration. Two growth factors were incorporated employing a multilayered coating based on polydopamine (PDA). In particular, Bone morphogenetic protein-2 (BMP-2) was bound onto the inner PDA layer while vascular endothelial growth factor (VEGF) was immobilized onto the outer one.
Herein, the in vitro release of both growth factors is evaluated. A fastest VEGF delivery followed by a slow and more sustained release of BMP-2 was demonstrated, thus fitting the needs for bone tissue engineering applications. Due to the relevance of the crosstalk between bone-promoting and vessel-forming cells during bone healing, the functionalized scaffolds are further assessed on a co-culture setup of human mesenchymal stem cells and human endothelial progenitor cells.
Osteogenic and angiogenic gene expression analysis indicates a synergistic effect between the growth factor-loaded scaffolds and the co-culture conditions. Taken together, these results indicate that the developed scaffolds hold great potential as an efficient platform for bone-tissue applications.
Language: | English |
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Publisher: | MDPI |
Year: | 2020 |
Pages: | 6418 |
ISSN: | 14220067 and 16616596 |
Types: | Journal article |
DOI: | 10.3390/ijms21176418 |
ORCIDs: | 0000-0002-7232-3998 , 0000-0002-2580-4641 , 0000-0002-3777-3205 , 0000-0002-6976-8283 and Hosta-Rigau, Leticia |
Angiogenesis Bone tissue engineering Co-delivery Growth factors Multilayers Osteogenesis Polydopamine Scaffolds
BMP2 protein, human Biology (General) Bone Morphogenetic Protein 2 Cells, Cultured Chemistry Coculture Techniques Endothelial Progenitor Cells Humans Indoles Mesenchymal Stem Cells Neovascularization, Physiologic Polymers QD1-999 QH301-705.5 Tissue Engineering Tissue Scaffolds VEGFA protein, human Vascular Endothelial Growth Factor A angiogenesis bone tissue engineering co-delivery growth factors multilayers osteogenesis polydopamine scaffolds