Journal article
High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging
University of Copenhagen1
Department of Biotechnology and Biomedicine, Technical University of Denmark2
Center for Microbial Secondary Metabolites, Centers, Technical University of Denmark3
Section for Microbial and Chemical Ecology, Department of Biotechnology and Biomedicine, Technical University of Denmark4
The crystal violet assay is widely used for biofilm quantitation despite its toxicity and variability. Here, we instead combine fluorescence labelling with the Cytation 5 multi-mode plate reader, to enable simultaneous acquisition of both quantitative and imaging biofilm data. This high-throughput method produces more robust data and provides information about morphology and spatial species organization within the biofilm.
| Language: | English |
|---|---|
| Year: | 2021 |
| Pages: | 106343 |
| ISSN: | 18728359 and 01677012 |
| Types: | Journal article |
| DOI: | 10.1016/j.mimet.2021.106343 |
| ORCIDs: | 0000-0002-0965-6837 , 0000-0003-1870-632X and Stannius, Rune Overlund |
