Journal article
ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR SCREENING OF MILK SAMPLES FOR SALMONELLA-TYPHIMURIUM IN DAIRY HERDS
We investigated the ability of an antibody-specific, O antigen-based ELISA to document Salmonella typhimurium herd infections by screening of milk samples. Three cattle populations, 20 herds with no history of salmonellosis, 8 herds with history of S typhimurium epsiodes within the previous 7 months, and 220 herds of unknown disease status, were tested.
A herd was considered ELISA positive if at least 5% of the cows had OD values > 0.3. Among the 20 herds without history of salmonellosis, only 2 herds were ELISA positive, whereas all 8 herds with a known history of salmonellosis were ELISA positive (herd specificity, 0.9 and herd sensitivity, 1.0).
A sig nificant correlation (P <0.001) was found between the OD values of serum and milk samples from cows in the herds with a history of salmonellosis. It was concluded that ELISA testing of individual milk sam ples can be used for surveillance of herds for S typhimurium infections, but further modifications are needed to test bulk tank milk samples.
Language: | English |
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Year: | 1995 |
Pages: | 1549-1554 |
ISSN: | 19435681 and 00029645 |
Types: | Journal article |
ORCIDs: | Hoorfar, Jeffrey |