Journal article
pH-rate profiles of L-arabinitol 4-dehydrogenase from Hypocrea jecorina and its application in L-xylulose production
Department of Chemical Engineering, Konkuk University, Seoul 143-701, Republic of Korea; Institute of SK-KU Biomaterials, Konkuk University, Seoul 143-701, Republic of Korea; Department of Environmental Engineering, Konkuk University, Seoul 143-701, Republic of Korea.1
Department of Chemical Engineering, Konkuk University, Seoul 143-701, Republic of Korea.2
Department of Biomedical Sciences, University of Ulsan, College of Medicine, Seoul 138-736, Republic of Korea.3
Institute of SK-KU Biomaterials, Konkuk University, Seoul 143-701, Republic of Korea; Department of Environmental Engineering, Konkuk University, Seoul 143-701, Republic of Korea. Electronic address: hankim@konkuk.ac.kr.4
Department of Chemical Engineering, Konkuk University, Seoul 143-701, Republic of Korea; Institute of SK-KU Biomaterials, Konkuk University, Seoul 143-701, Republic of Korea. Electronic address: jkrhee@konkuk.ac.kr.5
l-Arabinitol 4-dehydrogenase (LAD) from Hypocrea jecorina (HjLAD) was cloned and overexpressed in Escherichia coli BL21 (DE3). The kinetics of l-arabinitol oxidation by NAD(+), catalyzed by HjLAD, was studied within the pH range of 7.0-9.5 at 25°C. The turnover number (kcat) and the catalytic efficiency (kcat/Km) were 4200min(-1) and 290mM(-1)min(-1), respectively.
HjLAD showed the highest turnover number and catalytic efficiency among all previously characterized LADs. In further application of HjLAD, rare l-sugar l-xylulose was produced by the enzymatic oxidation of arabinitol to give a yield of approximately 86%.
| Language: | English |
|---|---|
| Year: | 2014 |
| Pages: | 173-176 |
| ISSN: | 14643405 and 0960894x |
| Types: | Journal article |
| DOI: | 10.1016/j.bmcl.2013.11.047 |
