Journal article
Towards On-site Pathogen Detection Using Antibody-based Sensors
Magnetic Systems Group, LabChip Section, Department of Micro- and Nanotechnology, Technical University of Denmark1
LabChip Section, Department of Micro- and Nanotechnology, Technical University of Denmark2
Department of Micro- and Nanotechnology, Technical University of Denmark3
Aarhus University4
In this paper the recent progress within biosensors for plant pathogen detection will be reviewed. Bio-recognition layers on sensors can be designed in various ways, however the most popular approach is to immobilise antibodies for specific capture of analytes. Focus will be put on antibody surface-immobilisation strategies as well as the use of antibodies in the widely used sensors, quartz crystal microbalance, surface plasmon resonance and cantilevers.
We will describe the available data on antibody-based plant pathogen detection and furthermore use examples from detection of the pathogens Salmonella, Listeria monocytogenes, Streptococcus mutans, Bacillus cereus, Bacillus anthracis, Campylobacter and Escherichia coli. We will touch upon optimal assay design and further discuss the strengths and limitations of current sensor technologies for detection of viruses, bacteria and fungi.
Language: | English |
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Year: | 2008 |
Pages: | 339-348 |
ISSN: | 18734235 and 09565663 |
Types: | Journal article |
DOI: | 10.1016/j.bios.2008.06.045 |
ORCIDs: | 0000-0001-7969-8760 |
Agriculture Antibodies, Bacterial Antibody Bacillus anthracis Bacillus cereus Bacteria Biomolecular interaction Biosensing Techniques Campylobacter Cantilever Enzyme-Linked Immunosorbent Assay Escherichia coli Former Faculty of Pharmaceutical Sciences Immunosensor Listeria monocytogenes Molecularly imprinted polymers Mycotoxin Pathogen Plants Point-of-Care Systems Point-of-care diagnostics QCM Quartz Real-time PCR SPR Salmonella Sensitivity and Specificity Streptococcus mutans Surface Plasmon Resonance