Journal article
Amplified fragment length polymorphism and pulsed field gel electrophoresis for subspecies differentiation of Serpulina pilosicoli
Management, National Veterinary Institute, Technical University of Denmark1
National Veterinary Institute, Technical University of Denmark2
Section for Veterinary Diagnostics, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark3
Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark4
Microbial Ecology, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark5
Danish Veterinary Laboratory6
Pulsed field gel electrophoresis (PFGE) and amplified fragment length polymorphism (AFLP) were compared for their ability to differentiate between 50 porcine Serpulina pilosicoli isolates. Both techniques were highly sensitive, dividing the isolates into 36 and 38 groups, respectively. Due to the low workload and high capacity and sensitivity of AFLP, this technique is recommended for future studies of S. pilosicoli.
S. pilosicoli isolates were genetically diverse, and generally identical subtypes were only identified in cultures from the same herd.
| Language: | English |
|---|---|
| Year: | 1999 |
| Pages: | 313-315 |
| ISSN: | 10958274 and 10759964 |
| Types: | Journal article |
| DOI: | 10.1006/anae.1999.0207 |
| ORCIDs: | Jensen, Tim Kåre and Boye, Mette |
