Journal article
Impedance Spectroscopic Characterisation of Porosity in 3D Cell Culture Scaffolds with Different Channel Networks
Department of Micro- and Nanotechnology, Technical University of Denmark1
Bioanalytics, Department of Micro- and Nanotechnology, Technical University of Denmark2
BioLabChip, Department of Micro- and Nanotechnology, Technical University of Denmark3
University of Oslo4
Fluidic Array Systems and Technology, Department of Micro- and Nanotechnology, Technical University of Denmark5
We present the application of electrochemical impedance spectroscopy (EIS) as a method for discriminating between different polydimethylsiloxane (PDMS) scaffolds for three-dimensional (3D) cell cultures. The validity of EIS characterisation for scaffolds having different degree of porosity (networks of structured or random channels) is discussed in relation to Archie's law.
Guidelines for EIS analysis are presented and demonstrated to provide porosity information in physiological buffer that agrees well with a more conventional weight-based approach. We also propose frequency ranges that may serve as means of single-frequency measurements for fast scaffold characterization combined with in vitro monitoring of 3D cell cultures.
Language: | English |
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Publisher: | WILEY‐VCH Verlag |
Year: | 2015 |
Pages: | 193-199 |
ISSN: | 15214109 and 10400397 |
Types: | Journal article |
DOI: | 10.1002/elan.201400413 |
ORCIDs: | Heiskanen, Arto , Dufva, Martin , Wolff, Anders and Emnéus, Jenny |