Journal article
Purification and characterization of xylitol dehydrogenase from Fusarium oxysporum
In
Biotechnology Letters
—
2002, Volume 24, Issue 24, pp. 2089-2092
Abstract
An NAD(+)-dependent xylitol dehydrogenase (XDH) from Fusarium oxysporum, a key enzyme in the conversion of xylose to ethanol, was purified to homogeneity and characterised. It was homodimeric with a subunit of M-r 48 000, and pI 3.6. It was optimally active at 45degreesC and pH 9-10. It was fully stable at pH 6-7 for 24 h and 30degreesC.
K-m values for D-xylitol and NAD(+) were 94 mM and 0.14 mM, respectively. Mn2+ at 10 mM increased XDH activity 2-fold and Cu2+ at 10 mM inhibited activity completely.
| Language: | English |
|---|---|
| Publisher: | Kluwer Academic Publishers |
| Year: | 2002 |
| Pages: | 2089-2092 |
| ISSN: | 15736776 and 01415492 |
| Types: | Journal article |
| DOI: | 10.1023/A:1021317614948 |
