Journal article
Prebiotic effect of xylooligosaccharides produced from birchwood xylan by a novel fungal GH11 xylanase
IPSBB Unit - Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.1
Instituto de Catálisis y Petroleoquímica, CSIC, Calle de Marie Curie, 2, 28049 Madrid, Spain.2
Biopolis S.L. Parc Científic Universitat de València, Catedrático Agustín Escardino, 9, 46980 Paterna, Spain.3
Lifesequencing S.L. Parque Científico Universitat de Valéncia, Catedrático Agustín Escardino, 9, 46980 Paterna, Spain.4
IPSBB Unit - Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain. Electronic address: mjmartinez@cib.csic.es.5
A fungal endoxylanase belonging to the glycoside hydrolase gene family 11 (GH11) was obtained from the ascomycete Talaromyces amestolkiae. The enzyme was purified, characterized and used to produce a mixture of xylooligosaccharides (XOS) from birchwood xylan. A notable yield of neutral XOS was obtained (28.8%) upon enzyme treatment and the mixture contained a negligible amount of xylose, having xylobiose, xylotriose and xylotetraose as its main components.
The prebiotic potential of this mixture was demonstrated upon analyzing the variations in microorganisms' composition and organic acids profile in breast-fed child faeces fermentations. The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides.
| Language: | English |
|---|---|
| Year: | 2017 |
| Pages: | 105-113 |
| ISSN: | 18737072 and 03088146 |
| Types: | Journal article |
| DOI: | 10.1016/j.foodchem.2017.03.149 |
