Journal article
Molecular ecology of macrolide-lincosamide-streptogramin B methylases in waste lagoons and subsurface waters associated with swine production
Department of Animal Sciences, University of Illinois at Urbana-Champaign, 1207 W. Gregory Dr., Urbana, IL 61801, USA.1
RNA methylase genes are common antibiotic resistance determinants for multiple drugs of the macrolide, lincosamide, and streptogramin B (MLS(B)) families. We used molecular methods to investigate the diversity, distribution, and abundance of MLS(B) methylases in waste lagoons and groundwater wells at two swine farms with a history of tylosin (a macrolide antibiotic structurally related to erythromycin) and tetracycline usage.
Phylogenetic analysis guided primer design for quantification of MLS(B) resistance genes found in tylosin-producing Streptomyces (tlr(B), tlr(D)) and commensal/pathogenic bacteria (erm(A), erm(B), erm(C), erm(F), erm(G), erm(Q)). The near absence of tlr genes at these sites suggested a lack of native antibiotic-producing organisms.
The gene combination erm(ABCF) was found in all lagoon samples analyzed. These four genes were also detected with high frequency in wells previously found to be contaminated by lagoon leakage. A weak correlation was found between the distribution of erm genes and previously reported patterns of tetracycline resistance determinants, suggesting that dissemination of these genes into the environment is not necessarily linked.
Considerations of gene origins in history (i.e., phylogeny) and gene distributions in the landscape provide a useful "molecular ecology" framework for studying environmental spread of antibiotic resistance.
Language: | English |
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Publisher: | Springer-Verlag |
Year: | 2010 |
Pages: | 487-498 |
ISSN: | 1432184x and 00953628 |
Types: | Journal article |
DOI: | 10.1007/s00248-009-9610-0 |
Animals DNA Primers DNA, Bacterial Drug Resistance, Multiple, Bacterial Ecology Fresh Water Genes, Bacterial Geoecology/Natural Processes Life Sciences Lincosamides Macrolides Microbial Ecology Microbiology Mobile Genetic Element Nature Conservation Phylogeny Polymerase Chain Reaction Sequence Analysis, DNA Streptogramin B Streptomyces Swine Swine Farm Swine Manure Tetracycline Resistance Tetracycline Resistance Gene Tylosin Waste Disposal, Fluid Water Microbiology tRNA Methyltransferases