Journal article
Production and partial characterization of arabinoxylan-degrading enzymes by Penicillium brasilianum under solid-state fermentation
The production of a battery of arabinoxylan-degrading enzymes by the fungus Penicillium brasilianum grown on brewer's spent grain (BSG) under solid-state fermentation was investigated. Initial moisture content, initial pH, temperature, and nitrogen source content were optimized to achieve maximum production of feruloyl esterase, xylanase, and alpha-L-arabinofuranosidase.
Under the optimum growth conditions (80% moisture, pH 6, 26.5 degrees C, and 5 g/l nitrogen source), the maximum level of feruloyl esterase (1,542 mU/g BSG) was found after 196 h, whereas xylanase (709 U/g BSG) and ArabF activity (3,567 mU/g BSG) were maximal after 108 h and 96 h, respectively. Based on substrate utilization data, the feruloyl esterases produced by P. brasilianum was anticipated to subclass B.
A crude enzyme (CE) preparation from P. brasilianum culture grown on BSG was tested for the release of hydroxycinnamic acids and pentoses from BSG. The P. brasilianum CE produced in this work contains a balance of cell wall-modifying enzymes capable of degrading arabinoxylan of BSG by more than 40%.
Language: | English |
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Publisher: | Springer Berlin Heidelberg |
Year: | 2006 |
Pages: | 1117-1124 |
ISSN: | 14320614 and 01757598 |
Types: | Journal article |
DOI: | 10.1007/s00253-006-0394-6 |
Biotechnology Carboxylic Ester Hydrolases Chemistry Coumaric Acids Edible Grain Endo-1,4-beta Xylanases Fermentation Freeze Drying Fungal Proteins Glycoside Hydrolases Humidity Hydrogen-Ion Concentration Hydroxycinnamic Acid Initial Moisture Content Microbial Genetics and Genomics Microbiology Nitrogen Compounds Penicillium Pentoses Substrate Specificity Temperature Time Factors Xylanase Activity Xylanase Production Xylans Xylose alpha-N-arabinofuranosidase arabinoxylan feruloyl esterase