Journal article
Characterisation of Toxoplasma gondii isolates using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) of the non-coding Toxoplasma gondii (TGR)-gene sequences
Section for Veterinary Epidemiology and public sector consultancy, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark1
Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark2
National Veterinary Institute, Technical University of Denmark3
The Toxoplasma gondii (TGR) genes constitute a family of non-coding sequences, three of which have been previously described as possible tools for typing of Toxoplasma gondii isolates. We obtained new isolates of T. gondii from domestic and wild animals, and used these to evaluate the possibility of using TGR gene variants as markers to distinguish among T. gondii isolates from different animals and different geographical sources.
Based on the band patterns obtained by restriction fragment length polymorphism (RFLP) analysis of the polymerase chain reaction (PCR) amplified TGR sequences, the T, gondii isolates could be separated into seven groups. Sequencing the amplified products showed that at least 20 TGR sequences not hitherto described had been found, demonstrating that the TGR gene family comprises a large number of different yet highly homologous sequences.
Each isolate had its own unique TCR sequence. The TGR gene family therefore seems a promising target for typing individual T. gondii isolates and for studying the genetic distance between two isolates, which can be used for tracing routes of infection.
Language: | English |
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Year: | 2000 |
Pages: | 853-858 |
ISSN: | 18790135 and 00207519 |
Types: | Journal article |
DOI: | 10.1016/S0020-7519(00)00059-X |
Animals Base Sequence Cats Cloning, Molecular DNA Primers DNA, Protozoan Denmark Deoxyribonucleases, Type II Site-Specific Electrophoresis, Polyacrylamide Gel Foxes GANTC-specific type II deoxyribonucleases Lagomorpha Molecular Sequence Data Phylogeny Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Sequence Alignment Sequence Analysis, DNA Sequence Homology, Nucleic Acid Sheep Swine Toxoplasma Toxoplasmosis, Animal