Journal article
Identification and characterization of a UDP-D-glucuronate 4-epimerase in ¤Arabidopsis¤
One of the major sugars present in the plant cell wall is d-galacturonate, the dominant monosaccharide in pectic polysaccharides. Previous work indicated that one of the activated precursors necessary for the synthesis of pectins is UDP-d-galacturonate, which is synthesized from UDP-d-glucuronate by a UDP-d-glucuronate 4-epimerase (GAE).
Here, we report the identification, cloning and characterization of a GAE6 from Arabidopsis thaliana. Functional analysis revealed that this enzyme converts UDP-d-glucuronate to UDP-d-galacturonate in vitro. An expression analysis of this epimerase and its five homologs in the Arabidopsis genome by quantitative RT-PCR and promoter::GUS fusions indicated differential expression of the family members in plant tissues and expression of all isoforms in the developing pollen of A. thaliana.
| Language: | English |
|---|---|
| Year: | 2004 |
| Pages: | 327-331 |
| ISSN: | 18733468 and 00145793 |
| Types: | Journal article |
| DOI: | 10.1016/j.febslet.2004.06.005 |
Arabidopsis Arabidopsis Proteins Base Sequence Carbohydrate Epimerases DNA Primers Flowers Kinetics Molecular Sequence Data Phylogeny Plant Leaves Plant Roots Plant Stems Polymerase Chain Reaction Recombinant Fusion Proteins Reverse Transcriptase Polymerase Chain Reaction UDPglucuronate 4-epimerase plant cell wallpectinnucleotide sugarepimerasecell-wall biosynthesisenzymatic-synthesisgalacturonic acidpectin synthesisplantsgenepurificationsensitivitythaliana
