Journal article
Topology optimized microbioreactors
Department of Chemical and Biochemical Engineering, Technical University of Denmark1
Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark2
Department of Systems Biology, Technical University of Denmark3
Department of Micro- and Nanotechnology, Technical University of Denmark4
Theoretical Microfluidics Group, Theory Section, Department of Micro- and Nanotechnology, Technical University of Denmark5
Theory Section, Department of Micro- and Nanotechnology, Technical University of Denmark6
This article presents the fusion of two hitherto unrelated fields—microbioreactors and topology optimization. The basis for this study is a rectangular microbioreactor with homogeneously distributed immobilized brewers yeast cells (Saccharomyces cerevisiae) that produce a recombinant protein. Topology optimization is then used to change the spatial distribution of cells in the reactor in order to optimize for maximal product flow out of the reactor.
This distribution accounts for potentially negative effects of, for example, by-product inhibition. We show that the theoretical improvement in productivity is at least fivefold compared with the homogeneous reactor. The improvements obtained by applying topology optimization are largest where either nutrition is scarce or inhibition effects are pronounced.
Language: | English |
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Publisher: | Wiley Subscription Services, Inc., A Wiley Company |
Year: | 2011 |
Pages: | 786-796 |
ISSN: | 10970290 and 00063592 |
Types: | Journal article |
DOI: | 10.1002/bit.23001 |
ORCIDs: | Eliasson Lantz, Anna , Bruus, Henrik and Gernaey, Krist |