Uncovering of Classical Swine Fever Virus adaptive response to vaccination by Next Generation Sequencing

Next Generation Sequencing (NGS) has rapidly become the preferred technology in nucleotide sequencing, and can be applied to unravel molecular adaptation of RNA viruses such as Classical Swine Fever Virus (CSFV). However, the detection of low frequency variants within viral populations by NGS is affected by errors introduced during sample preparation and sequencing, and so far no definitive solution to this problem has been presented.