Journal article
Quantitative determination of 64Cu-liposome accumulation at inflammatory and infectious sites: Potential for future theranostic system
University of Copenhagen1
Lund University2
Department of Health Technology, Technical University of Denmark3
Biotherapeutic Engineering and Drug Targeting, Department of Health Technology, Technical University of Denmark4
Colloids & Biological Interfaces, Biotherapeutic Engineering and Drug Targeting, Department of Health Technology, Technical University of Denmark5
Targeted Radionuclids, Hevesy and Dosimetry, Department of Health Technology, Technical University of Denmark6
Medical Isotopes, Hevesy and Dosimetry, Department of Health Technology, Technical University of Denmark7
Background : Therapeutic interventions for infectious and inflammatory diseases are becoming increasingly challenging in terms of therapeutic resistance and side-effects. Theranostic systems to ameliorate diagnosis and therapy are therefore highly warranted. The pathophysiological changes in inflammatory lesions provide an attractive basis for extravasation and accumulation of PEGylated liposomes.
The objective of this study was to provide direct quantitative information on the theranostic potential of radiolabeled liposome for accumulation in inflammatory models using position emission tomography (PET).. Method : Preclinical murine models of inflammation (turpentine and LPS), infection (Staphylococcus aureus) and collagen-induced arthritis (CIA) was established and monitored using bioluminescence imaging (BLI).
Across all models PET imaging using radiolabeled PEGylated liposomes (64Cu-liposomes) were performed and evaluated in terms of accumulation properties in inflammatory and infectious lesions.. Results : BLI demonstrated that the inflammatory and infectious models were successfully established and provided information on lesion pathology.
Activity of 64Cu-liposomes were increased in inflammatory and infectious lesions between early (10-minute or 3-hour) and late (24-hour) PET scans, which validates that a continuous extravasation and accumulation of long circulation PEGylated liposomes occurs.. Conclusion : The theranostic potential of long circulating PEGylated radiolabeled liposomes was shown in multiple preclinical models.
Impressive accumulation was seen in both inflammatory and infectious lesions. These results are encouraging towards advancing PEGylated liposomes as imaging and drug delivery systems in inflammatory and infectious diseases..
Language: | English |
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Year: | 2020 |
Pages: | 737-746 |
ISSN: | 01683659 and 18734995 |
Types: | Journal article |
DOI: | 10.1016/j.jconrel.2020.09.018 |
ORCIDs: | 0000-0002-2706-5547 , Henriksen, Jonas Rosager , Jensen, Andreas I. , Hansen, Anders Elias , Andresen, Thomas Lars and 0000-0001-9932-5996 |
ARTHRITIS Cell Line, Tumor ENHANCED PERMEABILITY Enhanced permeability and retention (EPR) effect GLUCOCORTICOIDS Infection Inflammation Liposomes MECHANISMS MOUSE MODEL Molecular imaging NANOPARTICLES OSTEOMYELITIS PHARMACOKINETICS Precision Medicine RADIOLABELED LIPOSOMES RETENTION Theranostic Nanomedicine