Journal article
Selection of Bacillus species for targeted in situ release of prebiotic galacto-rhamnogalacturonan from potato pulp in piglets
Department of Biotechnology and Biomedicine, Technical University of Denmark1
Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark2
Infection Microbiology, Section for Microbial and Chemical Ecology, Department of Biotechnology and Biomedicine, Technical University of Denmark3
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark4
Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark5
Bacterial Signal Transduction, Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark6
National Veterinary Institute, Technical University of Denmark7
Bacteriology & Parasitology, Division for Diagnostics & Scientific Advice, National Veterinary Institute, Technical University of Denmark8
Chr. Hansen AS9
Department of Chemical and Biochemical Engineering, Technical University of Denmark10
...and 0 moreWe have previously shown that galacto-rhamnogalacturonan fibers can be enzymatically extracted from potato pulp and that these fibers have potential for exerting a prebiotic effect in piglets. The spore-forming Bacillus species are widely used as probiotics in feed supplements for pigs. In this study, we evaluated the option for further functionalizing Bacillus feed supplements by selecting strains possessing the enzymes required for extraction of the potentially prebiotic fibers.
We established that it would require production and secretion of pectin lyase and/or polygalacturonase but no or limited secretion of galactanase and β-galactosidase. By screening a library of 158 Bacillus species isolated from feces and soil, we demonstrated that especially strains of Bacillus amyloliquefaciens, Bacillus subtilis, and Bacillus mojavensis have the necessary enzyme profile and thus the capability to degrade polygalacturonan.
Using an in vitro porcine gastrointestinal model system, we revealed that specifically strains of B. mojavensis were able to efficiently release galacto-rhamnogalacturonan from potato pulp under simulated gastrointestinal conditions. The work thus demonstrated the feasibility of producing prebiotic fibers via a feed containing Bacillus spores and potato pulp and identified candidates for future in vivo evaluation in piglets.
Language: | English |
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Publisher: | Springer Berlin Heidelberg |
Year: | 2017 |
Pages: | 3605-3615 |
ISSN: | 14320614 and 01757598 |
Types: | Journal article |
DOI: | 10.1007/s00253-017-8176-x |
ORCIDs: | Jers, Carsten , Strube, Mikael Lenz , Boye, Mette and Meyer, Anne S. |