Journal article
Combining Headspace Solid-Phase Microextraction with Internal Benchmarking to Determine the Elimination Kinetics of Hydrophobic UVCBs
Stockholm University1
Department of Environmental Engineering, Technical University of Denmark2
Circularity & Environmental Impact, Department of Environmental Engineering, Technical University of Denmark3
Environmental Fate & Effect of Chemicals, Department of Environmental Engineering, Technical University of Denmark4
Substances classified as unknown or variable composition, complex reaction products or biological origin (UVCB) present a challenge for environmental hazard and risk assessment. Here, we present a novel approach for whole-substance bioconcentration testing applied to cedarwood oil - an essential oil composed of volatile, hydrophobic organic chemicals.
The method yields whole-body elimination rate constants for a mixture of constituents. Our approach combines in vivo dietary fish exposure with internal benchmarking and headspace solid-phase microextraction (HS-SPME) equilibrium sampling followed by suspect-screening analysis. We quantified depuration rate constants of 13 individual cedarwood oil constituents based on relative peak areas using gas chromatography (GC) coupled with Orbitrap-mass spectrometry (MS) and GC triple-quadrupole (QqQ)-MS.
For seven constituents with available analytical standards, we compared the rate constants to the results obtained from solvent extraction, clean-up, and targeted GC-MS analysis. The HS-SPME sampling approach allowed for automated sample extraction and analyte enrichment while minimizing evaporative losses of the volatile target analytes and reducing matrix interferences from low-volatility organics.
The suspect-screening analysis enabled the quantification of constituents without available analytical standards, while the internal benchmarking significantly reduced variability from differences in delivered dose and analytical variability between the samples.
Language: | English |
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Publisher: | American Chemical Society |
Year: | 2021 |
Pages: | 11125-11132 |
ISSN: | 15205851 and 0013936x |
Types: | Journal article |
DOI: | 10.1021/acs.est.1c00179 |
ORCIDs: | Knudsmark Sjøholm, Karina , Mayer, Philipp , 0000-0002-7285-8044 and 0000-0003-2562-7339 |