Journal article
Draft genome sequence and chemical profiling of Fusarium langsethiae, an emerging producer of type A trichothecenes
Norwegian Institute of Bioeconomy Research1
Department of Systems Biology, Technical University of Denmark2
Eucaryotic Molecular Cell Biology, Department of Systems Biology, Technical University of Denmark3
Norwegian Veterinary Institute4
Consiglio per la Ricerca e la sperimentazione in Agricoltura5
Swedish University of Agricultural Sciences6
Metabolomics Platform, Department of Systems Biology, Technical University of Denmark7
Fusarium langsethiae is a widespread pathogen of small grain cereals, causing problems with T-2 and HT-2 toxin contamination in grains every year. In an effort to better understand the biology of this fungus, we present a draft genome sequence of F. langsethiae Fl201059 isolated from oats in Norway.
The assembly was fragmented, but reveals a genome of approximately 37.5 Mb, with a GC content around 48%, and 12,232 predicted protein-coding genes. Focusing on secondary metabolism we identified candidate genes for 12 polyketide synthases, 13 non-ribosomal peptide synthetases, and 22 genes for terpene/isoprenoid biosynthesis.
Some of these were found to be unique compared to sequence databases. The identified putative Tri5 cluster was highly syntenic to the cluster reported in F. sporotrichioides. Fusarium langsethiae Fl201059 produces a high number of secondary metabolites on Yeast Extract Sucrose (YES) agar medium, dominated by type A trichothecenes.
Interestingly we found production of glucosylated HT-2 toxin (Glu-HT-2), previously suggested to be formed by the host plant and not by the fungus itself. In greenhouse inoculations of F. langsethiae Fl201059 on barley and oats, we detected the type A trichothecenes: neosolaniol, HT-2 toxin, T-2 toxin, Glu-HT-2 and numerous derivatives of these.
Language: | English |
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Year: | 2016 |
Pages: | 29-36 |
ISSN: | 18793460 and 01681605 |
Types: | Journal article |
DOI: | 10.1016/j.ijfoodmicro.2016.01.008 |
ORCIDs: | 0000-0002-8006-6805 , Frandsen, Rasmus John Normand , Nielsen, Kristian Fog and Thrane, Ulf |
Cereals Food Science Fusarium langsethiae Genome Glucosylated mycotoxins HT-2 toxin Microbiology Oats Safety, Risk, Reliability and Quality Secondary metabolism T-2 toxin
Base Sequence Edible Grain Food Microbiology Fusarium Genome, Fungal Norway Trichothecenes