Journal article
Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coli
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellón 2, Av. Intendente Güiraldes 2160, C1428EGA Buenos Aires, Argentina.1
Bioreactor cultures of Escherichia coli recombinants carrying phaBAC and phaP of Azotobacter sp. FA8 grown on glycerol under low-agitation conditions accumulated more poly(3-hydroxybutyrate) (PHB) and ethanol than at high agitation, while in glucose cultures, low agitation led to a decrease in PHB formation.
Cells produced smaller amounts of acids from glycerol than from glucose. Glycerol batch cultures stirred at 125 rpm accumulated, in 24 h, 30.1% (wt/wt) PHB with a relative molecular mass of 1.9 MDa, close to that of PHB obtained using glucose.
Language: | English |
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Publisher: | American Society for Microbiology (ASM) |
Year: | 2010 |
Pages: | 2036-2040 |
ISSN: | 10985336 and 00992240 |
Types: | Journal article |
DOI: | 10.1128/AEM.02706-09 |