Journal article
Black perithecial pigmentation in Fusarium species is due to the accumulation of 5-deoxybostrycoidin-based melanin
Department of Systems Biology, Technical University of Denmark1
Eucaryotic Molecular Cell Biology, Department of Systems Biology, Technical University of Denmark2
Norwegian Veterinary Institute3
University of Oslo4
Norwegian Institute of Bioeconomy Research5
Department of Chemistry, Technical University of Denmark6
Organic Chemistry, Department of Chemistry, Technical University of Denmark7
Aalborg University8
Biosynthesis of the black perithecial pigment in the filamentous fungus Fusarium graminearum is dependent on the polyketide synthase PGL1 (oPKS3). A seven-membered PGL1 gene cluster was identified by over-expression of the cluster specific transcription factor pglR. Targeted gene replacement showed that PGL1, pglJ, pglM and pglV were essential for the production of the perithecial pigment.
Over-expression of PGL1 resulted in the production of 6-O-demethyl-5-deoxybostrycoidin (1), 5-deoxybostrycoidin (2), and three novel compounds 5-deoxybostrycoidin anthrone (3), 6-O-demethyl-5-deoxybostrycoidin anthrone (4) and purpurfusarin (5). The novel dimeric bostrycoidin purpurfusarin (5) was found to inhibit the growth of Candida albicans with an IC50 of 8.0 +/-1.9 mu M.
The results show that Fusarium species with black perithecia have a previously undescribed form of 5-deoxybostrycoidin based melanin in their fruiting bodies.
| Language: | English |
|---|---|
| Publisher: | Nature Publishing Group |
| Year: | 2016 |
| Pages: | 26206 |
| ISSN: | 20452322 |
| Types: | Journal article |
| DOI: | 10.1038/srep26206 |
| ORCIDs: | Frandsen, Rasmus John Normand , Gotfredsen, Charlotte Held and Larsen, Thomas Ostenfeld |
