Journal article
A Fragment of Collagen Type VI alpha-3 chain is Elevated in Serum from Patients with Gastrointestinal Disorders
Protease Network Degradomics, Section for Protein Science and Biotherapeutics, Department of Biotechnology and Biomedicine, Technical University of Denmark1
Department of Biotechnology and Biomedicine, Technical University of Denmark2
Nordic Bioscience AS3
Technical University of Denmark4
University of Pavia5
University College London6
Bispebjerg University Hospital7
University of Southern Denmark8
Extracellular matrix (ECM) remodeling is a hallmark of the pathology of gastrointestinal disorders. Collagen type VI (COL6) is produced by fibroblasts, and the COL6 α3-chain has shown to be elevated in patients with ulcerative colitis (UC), Crohn's disease (CD) and colorectal cancer (CRC). Measuring COL6α3 in serum may therefore have potential as a biomarker for gastrointestinal disorders.
The aims of this study were to develop and validate a competitive ELISA targeting a specific neo-epitope of COL6α3 and evaluate its associations with the gastrointestinal disorders UC, CD and CRC, in comparison to healthy controls. A monoclonal antibody was raised against a matrix metalloproteinase-2 and -9 specific cleavage site of COL6α3 (C6Mα3) and employed in a competitive enzyme-linked immunosorbent assay (ELISA).
The assay was developed and technically validated. Levels of C6Mα3 were measured in serum from patients with UC (n = 58), CD (n = 44) and CRC (n = 39) and compared to healthy controls (n = 32). The levels of C6Mα3 were elevated in patients with UC, CD and CRC patients compared to healthy controls (all p
| Language: | English |
|---|---|
| Publisher: | Nature Publishing Group UK |
| Year: | 2020 |
| Pages: | 5910 |
| ISSN: | 20452322 |
| Types: | Journal article |
| DOI: | 10.1038/s41598-020-62474-1 |
| ORCIDs: | 0000-0002-4612-1014 and 0000-0003-1642-7482 |
