Journal article
Collaborative study - Quantification of total folate in food using an efficient single-enzyme extraction combined with LC-MS/MS
National Food Institute, Technical University of Denmark1
Technical University of Munich2
Research Group for Bioactives – Analysis and Application, National Food Institute, Technical University of Denmark3
Institute of Marine Research4
Swedish Food Agency5
University of Helsinki6
Nestle7
Eurofins8
AQUANAL Laboratory9
Instituto Nacional de Saúde Doutor Ricardo Jorge10
...and 0 moreQuantification of the specific folate vitamers to estimate total folate in foods is not standardized. A collaborative study, including eight European laboratories, was conducted in order to determine the repeatability and reproducibility of the method for folate quantification in foods using the plant-origin γ-glutamyl hydrolase as part of the extraction procedure.
The seven food samples analyzed represent the food groups; fruits, vegetables, dairy products, legumes, offal, fish, and fortified infant formula. The homogenization step was included, and six folate vitamers were analyzed using LC-MS/MS. Total folate content, expressed as folic acid equivalent, was 17–490 μg/100 g in all samples.
Horwitz ratio values were within the acceptable range (0.60–1.94), except for fish. The results for fortified infant formula, a certified reference material (NIST 1869), confirmed the trueness of the method. The collaborative study is part of a standardization project within the Nordic Committee on Food Analysis (NMKL).
| Language: | English |
|---|---|
| Year: | 2020 |
| Pages: | 127447 |
| ISSN: | 03088146 and 18737072 |
| Types: | Journal article |
| DOI: | 10.1016/j.foodchem.2020.127447 |
| ORCIDs: | Ložnjak Švarc, Petra , 0000-0003-2206-1480 , 0000-0003-0207-2422 , 0000-0002-4591-0820 , 0000-0002-8382-312X , 0000-0002-5826-6288 and Jakobsen, Jette |
10-Formylfolic acid (PubChem CID: 135405023) 5,10-Methenyltetrahydrofolate (PubChem CID: 135398657) 5-Formyltetrahydrofolate (PubChem CID: 135402009) 5-Methyltetrahydrofolate (PubChem CID: 135415868) Deconjugase Folic acid (PubChem CID: 135398658) Homogenization Inter-laboratory study Plant enzyme Tetrahydrofolate (PubChem CID: 135444742) Tetrahydrofolates
