Journal article
Size and surface charge characterization of nanoparticles with a salt gradient
Nanofluidics and Bioimaging, Nano and Bio-physical Systems, Department of Health Technology, Technical University of Denmark1
Nano and Bio-physical Systems, Department of Health Technology, Technical University of Denmark2
Department of Health Technology, Technical University of Denmark3
Stochastic Systems and Signals, Nano and Bio-physical Systems, Department of Health Technology, Technical University of Denmark4
Department of Physics, Technical University of Denmark5
Exosomes are nanometer-sized lipid vesicles present in liquid biopsies and used as biomarkers for several diseases including cancer, Alzheimer’s, and central nervous system diseases. Purification and subsequent size and surface characterization are essential to exosome-based diagnostics. Sample purification is, however, time consuming and potentially damaging, and no current method gives the size and zeta potential from a single measurement.
Here, we concentrate exosomes from a dilute solution and measure their size and zeta potential in a one-step measurement with a salt gradient in a capillary channel. The salt gradient causes oppositely directed particle and fluid transport that trap particles. Within minutes, the particle concentration increases more than two orders of magnitude.
A fit to the spatial distribution of a single or an ensemble of exosomes returns both their size and surface charge. Our method is applicable for other types of nanoparticles. The capillary is fabricated in a low-cost polymer device.
Language: | English |
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Publisher: | Nature Publishing Group UK |
Year: | 2020 |
Pages: | 2337 |
ISSN: | 20411723 |
Types: | Journal article |
DOI: | 10.1038/s41467-020-15889-3 |
ORCIDs: | Rasmussen, Martin K. , Pedersen, Jonas N. and Marie, Rodolphe |