Journal article
High throughput production of mouse monoclonal antibodies using antigen microarrays
Recent advances in proteomics research underscore the increasing need for high-affinity monoclonal antibodies, which are still generated with lengthy, low-throughput antibody production techniques. Here we present a semi-automated, high-throughput method of hybridoma generation and identification. Monoclonal antibodies were raised to different targets in single batch runs of 6-10 wk using multiplexed immunisations, automated fusion and cell-culture, and a novel antigen-coated microarray-screening assay.
In a large-scale experiment, where eight mice were immunized with ten antigens each, we generated monoclonal antibodies against 68 of the targets (85%), within 6 wk of the primary immunization.
Language: | English |
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Publisher: | WILEY-VCH Verlag |
Year: | 2005 |
Pages: | 4070-4081 |
ISSN: | 16159861 and 16159853 |
Types: | Journal article |
DOI: | 10.1002/pmic.200401279 |
ORCIDs: | De Masi, Federico |