Journal article
Identification of DNA-binding sites for the activator involved in late transcription of the temperate lactococcal phage TP901-1
Alt, encoded by the lactococcal phage TP901-1, is needed for late transcription. We identify Alt as a DNA-binding protein, and footprint analysis shows that Alt binds to a region containing four imperfect direct repeats (ALT boxes) located -76 to -32 relative to the P-late transcriptional start site.
The importance of the ALT boxes was confirmed by deletion of one or two ALT boxes and by introducing mutations in ALT boxes 1 and 4. Alt is proposed to act as a tetramer or higher multimer activating transcription of TP901-1 late genes by binding to the four ALT boxes, and bending of the DNA may be important for transcriptional activation of P-late.
Furthermore, our results suggest that DNA replication may be required for late transcription in TP901-1. Additionally, we identify gp28 of the related lactococcal phage Tuc2009 as an activator and show that the activators required for late transcription in TP901-1 and Tuc2009 are interchangeable. (c) 2005 Elsevier Inc.
All rights reserved.
| Language: | English |
|---|---|
| Year: | 2006 |
| Pages: | 446-456 |
| ISSN: | 10960341 , 1089862x and 00426822 |
| Types: | Journal article |
| DOI: | 10.1016/j.virol.2005.10.007 |
| ORCIDs: | Kilstrup, Mogens |
Activator Amino Acid Sequence Bacteriophage TP901-1 Base Sequence Binding Sites DNA Footprinting DNA-Binding Proteins DNA-binding protein Direct repeats Footprint Gene Expression Regulation, Viral Lactococcus lactis Late transcription Molecular Sequence Data Siphoviridae Transcription, Genetic Transcriptional Activation Viral Proteins
