Journal article
Subacute oral toxicity investigation of nanoparticulate and ionic silver in rats
Division of Toxicology and Risk Assessment, National Food Institute, Technical University of Denmark1
National Food Institute, Technical University of Denmark2
Division of Food Chemistry, National Food Institute, Technical University of Denmark3
Division of Microbiology and Risk Assessment, National Food Institute, Technical University of Denmark4
Chinese Academy of Sciences5
DHI Water - Environment - Health6
Subacute toxicity of 14 nm nanoparticulate silver (Ag-NP) stabilised with polyvinylpyrrolidone and ionic silver in the form of silver acetate (Ag-acetate) was investigated in four-week-old Wistar rats. Animals received orally by gavage the following: vehicle control (10 $, 6 #); Ag-NP at doses: 2.25 (8 $), 4.5 (8 $) or 9 mg/kg bw/day (10 $, 6 #); or Ag-acetate 9 mg silver/kg bw/day (8 $) for 28 days.
Clinical, haematolological and biochemical parameters, organ weights, macro- and microscopic pathological changes were investigated. Caecal bacterial phyla and their silver resistance genes were quantified. For the Ag-NP groups, no toxicological effects were recorded. For Ag-acetate, lower body weight gain (day 4–7, 11–14, 14–16, P\0.05; overall, day 1–28, P\0.01), increased plasma alkaline phosphatase (P\0.05), decreased plasma urea (P\0.05) and lower absolute (P\0.01) and relative (P\0.05) thymus weight were recorded.
In conclusion, these findings indicate toxicity of 9 mg/kg bw/day ionic silver but not of an equimolar Ag- NP dose. This is in accordance with previously reported data showing that oral Ag-acetate, in comparison with an equimolar dose of Ag-NP, resulted in higher silver plasma and organ concentrations.
Language: | English |
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Publisher: | Springer-Verlag |
Year: | 2012 |
Pages: | 543-551 |
ISSN: | 14320738 and 03405761 |
Types: | Journal article |
DOI: | 10.1007/s00204-011-0759-1 |
ORCIDs: | Hadrup, Niels , Löschner, Katrin , Bergström, Anders and Frandsen, Henrik Lauritz |
Acetates Administration, Oral Animals Biomedicine Biomedicine general Clinical Chemistry Tests Dose-Response Relationship, Drug Environmental Health Female Hematologic Tests Ions Male Metal Nanoparticles Nanocomposites Nanoparticle No-Observed-Adverse-Effect Level Occupational Medicine/Industrial Medicine Particle Size Pharmacology/Toxicology Povidone Rat Rats Rats, Wistar Silver Silver Compounds Specific Pathogen-Free Organisms Subacute Toxicity Tests Toxicology silver acetate