Journal article
Tissue-based map of the human proteome
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark1
High Throughput Molecular Bioscience, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark2
KTH Royal Institute of Technology3
Uppsala University4
Chalmers University of Technology5
TU Dortmund University6
Lab Surgpath7
Karolinska Institutet8
Stockholm University9
Resolving the molecular details of proteome variation in the different tissues and organs of the human body will greatly increase our knowledge of human biology and disease. Here, we present a map of the human tissue proteome based on an integrated omics approach that involves quantitative transcriptomics at the tissue and organ level, combined with tissue microarray-based immunohistochemistry, to achieve spatial localization of proteins down to the single-cell level.
Our tissue-based analysis detected more than 90% of the putative protein-coding genes. We used this approach to explore the human secretome, the membrane proteome, the druggable proteome, the cancer proteome, and the metabolic functions in 32 different tissues and organs. All the data are integrated in an interactive Web-based database that allows exploration of individual proteins, as well as navigation of global expression patterns, in all major tissues and organs in the human body.
Language: | English |
---|---|
Publisher: | American Association for the Advancement of Science |
Year: | 2015 |
Pages: | 1260419 |
ISSN: | 10959203 and 00368075 |
Types: | Journal article |
DOI: | 10.1126/science.1260419 |