Journal article
Phylogenetic characterisation of the G(L) sequences of equine arteritis virus isolated from semen of asymptomatic stallions and fatal cases of equine viral arteritis in Denmark
Virology, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark1
Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark2
National Veterinary Institute, Technical University of Denmark3
Section for Veterinary Diagnostics, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark4
The study describes for the first time the phylogenetic relationship between equine arteritis virus (EAV) isolated from asymptomatic virus-shedding stallions and fatal cases of equine viral arteritis (EVA) in an European country. EAV was isolated from three dead foals and an aborted foetus during three different outbreaks of EVA.
From these fatalities, the complete open reading frame 5, encoding the EAV GL protein, was amplified by reverse transcription-polymerase chain reaction and subjected to nucleotide sequence analysis. Furthermore, DNA sequences were obtained from virus isolated from semen samples of seven virus-shedding, but clinically healthy, Danish stallions.
DNA sequence alignment revealed an overall divergence of 0-14 and 0-10% at the nucleotide and amino acid levels, respectively. Phylogenetic analysis including 24 previously published sequences revealed that European as well as North American "types" of EAV were present in the semen of asymptomatic carrier stallions and in fatal cases of EVA.
Our results reveal that the presence of EAV-shedding stallions in Denmark represents a potential source of severe EVA.
| Language: | English |
|---|---|
| Year: | 2001 |
| Pages: | 339-346 |
| ISSN: | 18732542 and 03781135 |
| Types: | Journal article |
| DOI: | 10.1016/S0378-1135(01)00323-6 |
| ORCIDs: | Larsen, Lars Erik |
Amino Acid Sequence Animals Arterivirus Infections Denmark Equartevirus Female Horse Diseases Horses Male Molecular Sequence Data Open Reading Frames Phylogeny Reverse Transcriptase Polymerase Chain Reaction Semen Sequence Alignment Sequence Analysis, DNA Viral Envelope Proteins Virus Shedding glycoprotein L, equine arteritis virus
