Journal article
Evaluation of an indirect enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to the Apx toxins of Actinobacillus pleuropneumoniae
Section for Veterinary Diagnostics, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark1
Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark2
National Veterinary Institute, Technical University of Denmark3
Secretariat, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark4
The reference strains of the 12 serotypes of Actinobacillus pleuropneumoniae express one or two of three different RTX exotoxins designated Apr I, Apr II and Apr III. The toxins are important virulence factors. In the present study, ELISAs with purified Apr I, Apr II and Apr III, respectively, as antigen were evaluated as candidates for serological diagnosis of Actinobacillus pleuropneumoniae infection in pigs, The pigs were inoculated with biotype 1, serotypes 1-12, and biotype 2, serotype 14, respectively.
A strong humoral antibody response was seen to all the three antigens in most pigs irrespective of the serotype used for inoculation. However, titers to the exotoxins secreted by the serotype used for inoculation were generally highest. The results show that toxin proteins of Actinobacillus pleuropneumoniae line are antigenically related and that a correlation between serotype and secretion of exotoxin is not revealed serologically in the ELISA test.
Language: | English |
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Year: | 2000 |
Pages: | 81-87 |
ISSN: | 18732542 and 03781135 |
Types: | Journal article |
DOI: | 10.1016/S0378-1135(99)00157-1 |
Actinobacillus Infections Animals Antibodies, Bacterial ApxI toxin, Bacteria ApxII toxin, bacteria ApxIII toxin protein, Actinobacillus Bacterial Proteins Bacterial Toxins Enzyme-Linked Immunosorbent Assay Hemolysin Proteins Immune Sera Pleuropneumonia Specific Pathogen-Free Organisms Swine Swine Diseases Virulence